synthetic gene asnb Search Results


synthetic gene asnb  (GenScript corporation)
90
GenScript corporation synthetic gene asnb
Synthetic Gene Asnb, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+gene+asnb/pm30990115-19-1-15?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
synthetic gene asnb - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
gene exp asns mm00803785 m1  (Thermo Fisher)
85
Thermo Fisher gene exp asns mm00803785 m1
Time- and dose-dependent correction of MCK- Fxn heart phenotype after AAV9-CAG-FXN treatment (A) Heart to body weight ratios at the time of necropsy: 57 days post-injection (dpi) for WT untreated (n = 15) and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 13), 14 dpi for MCK- Fxn saline group (n = 12), 26–57 dpi for MCK- Fxn treated at 3 × 10 13 vg/kg (n = 11), and 20–26 dpi for MCK- Fxn treated at 1 × 10 14 vg/kg (n = 12). (B) Vector genome copy (VGC) of AAV9-CAG-FXN in heart samples, as measured by ddPCR at the time of necropsy in WT untreated (n = 15), MCK- Fxn saline (n = 12), and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 12), 3 × 10 13 vg/kg (n = 10), or 1 × 10 14 vg/kg (n = 11). (C) mRNA expression of endogenous mouse Fxn and human FXN transgene in heart samples, as measured by ddPCR in WT untreated (n = 14), MCK- Fxn saline (n = 11), and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 13), 3 × 10 13 vg/kg (n = 11), or 1 × 10 14 vg/kg (n = 11). (D) Detection of Fxn transgene mRNA expression by in situ hybridization (ISH, upper panels) and human FXN protein by immunohistochemistry (IHC, lower panels) in heart sections. Scale bars, 300 μm. (E) Western blot analysis of mitochondrial and Fe-S client proteins from three mouse heart extracts from each group. Vinculin (VINC) was used as a loading control. (F) mRNA expression of cardiac dysfunction and FXN deficiency markers Nppa , <t>Asns</t> , Mthfd2 , and Gdf15 as measured by ddPCR in WT untreated (n = 15), MCK- Fxn saline (n = 11), and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 13), 3 × 10 13 vg/kg (n = 11), or 1 × 10 14 vg/kg (n = 12). All data are mean ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
Gene Exp Asns Mm00803785 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+gene+asnb/pmc08866050-270-35-45?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp asns mm00803785 m1 - by Bioz Stars, 2026-07
85/100 stars
  Buy from Supplier
human asns asparagine synthetase gene orf cdna clone in cloning vector  (Sino Biological)
N/A
Full length Clone DNA of Human asparagine synthetase glutamine hydrolyzing
   Buy from Supplier
ntan1 antibody  (ProSci Incorporated)
N/A
NTAN1 Antibody raised in Rabbit validated in WB in Human.
   Buy from Supplier
fasn antibody  (ProSci Incorporated)
N/A
FASN Antibody raised in Rabbit validated in WB, IHC-P, IF, Flow in Human.
   Buy from Supplier
human asparagine synthetase asns activation kit by crispra  (OriGene)
N/A
ASNS CRISPRa kit CRISPR gene activation of human asparagine synthetase glutamine hydrolyzing
   Buy from Supplier
asparagine synthetase asns human qpcr template standard  (OriGene)
N/A
qPCR primer pairs and template standards against Homo sapiens gene ASNS
   Buy from Supplier
asns antibody  (ProSci Incorporated)
N/A
ASNS Antibody raised in Rabbit validated in WB in Human, Mouse.
   Buy from Supplier
asnsd1 antibody  (ProSci Incorporated)
N/A
ASNSD1 Antibody raised in Rabbit validated in WB in Human.
   Buy from Supplier
human asns asparagine synthetase gene lentiviral orf cdna expression plasmid c gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Homo sapiens asparagine synthetase glutamine hydrolyzing
   Buy from Supplier
fatty acid synthase fasn antibody 497ct15 2 5 unpurified  (Novus Biologicals)
N/A
The Fatty Acid Synthase FASN Antibody 497CT15 2 5 Unpurified from Novus Biologicals is a mouse monoclonal antibody to Fatty Acid Synthase FASN This antibody reacts with human mouse The Fatty Acid Synthase FASN Antibody
   Buy from Supplier

Image Search Results


Time- and dose-dependent correction of MCK- Fxn heart phenotype after AAV9-CAG-FXN treatment (A) Heart to body weight ratios at the time of necropsy: 57 days post-injection (dpi) for WT untreated (n = 15) and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 13), 14 dpi for MCK- Fxn saline group (n = 12), 26–57 dpi for MCK- Fxn treated at 3 × 10 13 vg/kg (n = 11), and 20–26 dpi for MCK- Fxn treated at 1 × 10 14 vg/kg (n = 12). (B) Vector genome copy (VGC) of AAV9-CAG-FXN in heart samples, as measured by ddPCR at the time of necropsy in WT untreated (n = 15), MCK- Fxn saline (n = 12), and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 12), 3 × 10 13 vg/kg (n = 10), or 1 × 10 14 vg/kg (n = 11). (C) mRNA expression of endogenous mouse Fxn and human FXN transgene in heart samples, as measured by ddPCR in WT untreated (n = 14), MCK- Fxn saline (n = 11), and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 13), 3 × 10 13 vg/kg (n = 11), or 1 × 10 14 vg/kg (n = 11). (D) Detection of Fxn transgene mRNA expression by in situ hybridization (ISH, upper panels) and human FXN protein by immunohistochemistry (IHC, lower panels) in heart sections. Scale bars, 300 μm. (E) Western blot analysis of mitochondrial and Fe-S client proteins from three mouse heart extracts from each group. Vinculin (VINC) was used as a loading control. (F) mRNA expression of cardiac dysfunction and FXN deficiency markers Nppa , Asns , Mthfd2 , and Gdf15 as measured by ddPCR in WT untreated (n = 15), MCK- Fxn saline (n = 11), and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 13), 3 × 10 13 vg/kg (n = 11), or 1 × 10 14 vg/kg (n = 12). All data are mean ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: In vivo overexpression of frataxin causes toxicity mediated by iron-sulfur cluster deficiency

doi: 10.1016/j.omtm.2022.02.002

Figure Lengend Snippet: Time- and dose-dependent correction of MCK- Fxn heart phenotype after AAV9-CAG-FXN treatment (A) Heart to body weight ratios at the time of necropsy: 57 days post-injection (dpi) for WT untreated (n = 15) and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 13), 14 dpi for MCK- Fxn saline group (n = 12), 26–57 dpi for MCK- Fxn treated at 3 × 10 13 vg/kg (n = 11), and 20–26 dpi for MCK- Fxn treated at 1 × 10 14 vg/kg (n = 12). (B) Vector genome copy (VGC) of AAV9-CAG-FXN in heart samples, as measured by ddPCR at the time of necropsy in WT untreated (n = 15), MCK- Fxn saline (n = 12), and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 12), 3 × 10 13 vg/kg (n = 10), or 1 × 10 14 vg/kg (n = 11). (C) mRNA expression of endogenous mouse Fxn and human FXN transgene in heart samples, as measured by ddPCR in WT untreated (n = 14), MCK- Fxn saline (n = 11), and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 13), 3 × 10 13 vg/kg (n = 11), or 1 × 10 14 vg/kg (n = 11). (D) Detection of Fxn transgene mRNA expression by in situ hybridization (ISH, upper panels) and human FXN protein by immunohistochemistry (IHC, lower panels) in heart sections. Scale bars, 300 μm. (E) Western blot analysis of mitochondrial and Fe-S client proteins from three mouse heart extracts from each group. Vinculin (VINC) was used as a loading control. (F) mRNA expression of cardiac dysfunction and FXN deficiency markers Nppa , Asns , Mthfd2 , and Gdf15 as measured by ddPCR in WT untreated (n = 15), MCK- Fxn saline (n = 11), and MCK- Fxn treated at 1 × 10 13 vg/kg (n = 13), 3 × 10 13 vg/kg (n = 11), or 1 × 10 14 vg/kg (n = 12). All data are mean ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: The following primer/probe sets were used: primer/probe set targeting the codon-optimized cDNA of WT and N146K human FXN was designed and synthesized (Applied Biosystems, Sparta, NJ); mouse Fxn (FAM20X, Applied Biosystems, #dMmuCPE5107916); Asns (FAM 20X, #Mm00803785_m1, Applied Biosysems, Foster City, CA); Nppa (FAM 20X, #Mm01255747_g1, Applied Biosystems); Mthfd2 (FAM 20X, #Mm00485276_m1, Applied Biosystems), and Gdf15 (FAM 20X, #Mm00442228_m1, Applied Biosystems); Gapdh (HEX 20X, #dMmuCPE5195283, Bio-Rad); Hprt (HEX 20X, #dMmuCPE5095493, Bio-Rad); and Tbp (VIC 20X, #Mm00446973_m1, Applied Biosystems); mouse Tfrc Copy Number Reference Assay (VIC-Tamara 20X) (#4458366, Applied Biosystems).

Techniques: Injection, Saline, Plasmid Preparation, Expressing, In Situ Hybridization, Immunohistochemistry, Western Blot, Control